Collectively these experiments indicate that lung epithelial stem cells are regionally specific.
Lung cell lineage.
The mammalian lung is a highly branched network in which the distal regions of the bronchial tree transform during development into a densely packed honeycomb of alveolar air sacs that mediate gas exchange.
In day 21 balo cultures upregulated genes included hopx for aec i and sftpc for aec ii as well as foxj1 muc5ac and p63 for ciliated goblet cells and basal cells respectively fig 2 a.
Clones x360 x8 and x232 are found contributing to multiple cell lineages multipotent whereas others contribute only to lung lineage x314 lung and pnec x401 and nle only x123.
At2 and at1 cells are thought to receive signals from the adjoining lung mesenchyme which are important for both homeostasis and regeneration after injury.
Rock et al 2011.
Lung lineage specific cell differentiation was achieved in a relatively shorter span of time 22 days in contrast to other reported lung lineage culture conditions.
E lentivirally barcoded cells projected onto the cshmm based using 86 selected genes.
Furthermore we validated upregulation of lineage markers of differentiated cell types of the adult lung by qpcr.
There is no evidence that a single lung stem cell as in the hematopoietic system gives rise to all cell lineages during homeostasis or repair.
In addition the cells must differentiate into at least 40 distinct lung cell lineages.
Single cell atlas of lung innate lymphoid cells.
Specific transcriptional factors peptide growth factor receptor mediated signaling pathways extracellular matrix components and integrin signaling pathways interact to direct lung morphogenesis and lung cell lineage differentiation.
To shed light on these unknown mechanisms scientists at the max planck institute of immunobiology and epigenetics in freiburg headed by dominic.
This reflects the fact that cell specific lineage tracing systems are better developed for the epithelium.
The lung alveolus forms a niche of interacting and communicating cell types including the at1 and at2 epithelial lineages vascular endothelial cells and poorly understood mesenchymal lineages.
The lung alveolar compartment is the primary site of gas exchange the most fundamental function of the respiratory system.
Reconstructing lineage hierarchies of the distal lung epithelium using single cell rna seq.
